Sekventering

The Small RNA-Seq Library Prep Kit provides reagents for generating small RNA libraries for Illumina sequencing directly from enriched small RNA.

CORALL enables streamlined generation of Illumina-compatible libraries within 4.5 hours, featuring seamless integration of Unique Molecular Identifiers (UMIs) and exceptional protocol-inherent strand specificity (>99 %). The fragmentation-free protocol uses Lexogen’s proprietary Strand Displacement Stop and Ligation technologies to deliver complete transcript representation, including start and end sites. All CORALL RNA-Seq V2 Kits and bundles are delivered with Lexogen UDI 12 nt Unique Dual Indexing Sets for Unique Dual Indexing.

The TeloPrime Full-Length cDNA Amplification Kit is an all-in-one protocol for generating full-length cDNA from total RNA.

The TeloPrime PCR Add-on Kit V2 contains 16 reactions of the PCR Mix, the polymerase and the Forward and Reverse Primer separately. Based on this primers can be easily exchanged with gene-specific primers. The kit can furthermore be used to run multiple PCR reactions of your sample to generate sufficient template for any downstream application.

TraPR (Trans-kingdom, rapid, affordable Purification of RISCs) presents a gel- and bias-free, column-based method for isolation of functional small RNAs from RNA-induced silencing complexes (RISCs) of all organisms.

The UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) (Cat. No. 081.96) contains the UMI Second Strand Synthesis Mix (USS). This mix simply replaces the Second Strand Synthesis Mix 1 (SS1) from the standard QuantSeq FWD Kit. The module allows unique tagging of individual transcripts with 6 nt long Unique Molecular Identifiers (UMI) located between the partial P5 adapter and the random priming sequence. indexing options

Yeast total RNA is comprised of large amounts of undesired ribosomal RNA (rRNA) which accounts for up to ~97 % of all transcripts. Lexogen’s RiboCop rRNA Depletion Kits for Yeast remove undesired cytoplasmic (25S, 18S, 5.8S, 5S, and 35S) and mitochondrial (21S, 15S, ACI60_gr01 and ACI60_gr02) rRNA from intact as well as degraded input RNA. Ribo-depletion affords an unbiased view of the transcriptome, including non-coding and non-polyadenylated RNA species.