Lexogen
SIRV-Set 1 (Iso Mix E0, E1, E2)
The SIRVs are a set of 69 artificial transcript variants which mimic 7 human model genes. They are complemented by additional isoforms to comprehensively reflect variations of alternative splicing, alternative transcription start- and end-sites, overlapping genes, and antisense transcripts.
The SIRVs are provided as a set of 3 SIRV mixes, E0, E1, and E2, with each mix containing all 69 SIRVs but in different concentration ratios. E0 contains the RNAs in equimolar ratio, E1 covers one order of magnitude (up to 1:8) and in E2 SIRV concentration range over more than two orders of magnitude (up to 1:128). The comparison of samples spiked with these different mixes enables the quantitative assessment of differential expression workflows on the transcript level.