Recombinant ACF Complex was expressed in Sf9 cells and supplied at a concentration of 0.5 μg/μl in 20 mM Tris pH 8.0, 300 mM KCl, 20% Glycerol, 0.2 mM EDTA.

ACF Complex (ATP-utilizing chromatin assembly and remodeling factor protein complex) which contains Acf1 and Iswi catalyzes the ATP-dependent assembly of periodic nucleosome arrays in vitro. The catalytic subunit of ACF is the ISWI ATP-dependant chromatin remodeling protein. ACF is related to CHRAC (Chromatin Accessibility Complex), which also contains Acf1 and Iswi. ACF is involved in the establishment and possibly maintenance of silenced heterochromatic domains in the genome, both at pericentric heterochromatin and at genes repressed by the Polycomb complex.