Zymolyase-20T is an enzyme preparation from a submerged culture of Arthrobacter luteus which effectively lyses cell walls of viable yeast cells. This preparation is a lyophilized powder prepared by salting out from the culture fluid with ammonium sulfate and contains 20,000 units enzyme/g. The primary enzyme in this preparation responsible for lysis of viable yeast cells is ß-1,3-glucan laminaripentaohydrolase. It hydrolyzes glucose polymers at the ß-1,3-glucan linkages releasing laminaripentaose as the principal product.

 

Zymolyase-100T is an enzyme preparation from a submerged culture of Arthrobacter luteus which effectively lyses cell walls of viable yeast cells. This preparation is lyophilized enzyme partially purified by affinity chromatography and contains 100,000 units enzyme/g. The primary enzyme in this preparation responsible for lysis of viable yeast cells in ß-1,3-glucan laminaripentaohydrolase. It hydrolyzes glucose polymers at the ß-1,3-glucan linkages releasing laminaripentaose as the principal product.