Biosearch Technologies (Lucigen)
Ready-Lyse Lysozyme Solution
Lyse Gram positive or Gram negative bacteria for protein or nucleic acid purifications from bacteria.
- Highly Active: This enzyme is 200X more active than egg white lysozyme, thus improving bacterial lysis and using less enzyme
- No sample agitation or heat generation necessary during lysis which helps preserve protein function
- Usuage volumes are easily adjusted based on the scale of the experiment
Unit Definition: One unit of Ready-Lyse Lysozyme produces a decrease in A350 of 0.001 per minute at 23°C with a 0.5 mg/ml suspension of lyophilized E. coli K802 cells in 50 mM Tris-HCl (pH 7.5).
Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.