There is a lysing bead for every tissue
Lyse the Toughest, Hardest and Most Difficult Samples with ease.
FastPrep® Lysing beads and matrices make difficult-to-lyse samples easy. No matter how tough or resistant your samples are our bead beating tubes will effectively disrupt cell walls, providing the highest yields of nucleic acids and proteins in a matter of seconds. Sample lysis tubes from MP Biomedicals are highly reproducible with no cross-contamination. All lysing matrix tubes are standard sizes and fit just about any homogenizer on the market. We offer a wide variety of lysing beads and matrices to fit all sample types and applications.
- Optimal cell disruption for any sample
- Size of the beads and composition optimized according to the sample
- No cross contamination with closed Lysing Matrix tubes
- Available in 2ml, 4.5ml, 15ml, 50ml tubes or 96 well plates
- Fit any high-speed bead-beating homogenizers
- Validated worldwide with 3,000+ Lysing Matrix specific publications
FastPrep® sample tubes range from low to high impaction breaking down any sample type whether the cell walls are hard and brittle or soft. Sample types include but are not limited to: human and animal and plant tissues; microorganisms like bacterial, yeast and fungi; plant, soil, fecal, plus insects and worms.
Impact-resistant lysis tubes with beads are available in 2 mL, 4.5 mL, 15 mL, 50 mL and 96-well format sizes and contain a wide variety of materials to meet your lysing, grinding, and homogenization needs. All matrix particles are produced to the highest quality standards to ensure optimum performance. The lysing matrix particles are then dispensed into the Lysing Matrix Tubes under a rigorous set of proprietary conditions allowing complete confidence for immediate use.
For optimal performance and results we recommend using in conjunction with our FastPrep-24™ 5G Homogenizer and FastPrep® Extraction Kits. Using this combination to easily grind, lyse and homogenize any sample type in seconds. Not only is it fast and efficient but can be dependable time over time.
FastPrep® Lysing Matrix tubes are very versatile, consisting of 16 unique sample tubes that fit any sample type! Whatever your sample, we have the solution. Our matrices contain properties that contribute to optimum success in your protocols. Various properties of a lysing matrix particle, whether inherent or selected, can yield a myriad of end-products depending on the dynamic qualities instilled in that particle by said properties. The inherent properties include the hardness, composition, and density, while the selected properties include the shape and the size.
What makes FastPrep® Lysing Beads Special?
The smaller the particles used in the grinding media, the smaller the average particle size and the smaller the lowest-limiting particle size produced during pulverization. Matrix particle size should be selected based upon the size of the particles you wish to obtain in your lysate. For example, if your goal is to obtain intact and functional organelles, it’s best to utilize a larger grinding media. Utilizing a smaller size matrix would be more conducive to lysing smaller structures, such as viral particles and endospores.
The shape of the grinding media is a major determining factor in how cells are disrupted. Dull media, such spherical beads, utilize cascade impaction (hammering) as the main force for cell lysis. Sharp and angular shaped media will primarily generate mechanical shear forces (chopping and cutting) which can quickly open difficult cell walls, grind fibrous or elastic animal tissue, or crack spores or oocytes. Shear forces are preferable when isolating stable molecules such as DNA, stable proteins, structural polysaccharides and small molecules or metabolites. RNA and certain easily denatured proteins can be quickly degraded by shear forces, so care needs to be taken when using angular media. For isolation of these molecules, smooth impactor grinding media can be much more forgiving.
HARDNESS, DENSITY, COMPOSITION
The composition determines two very important qualities: hardness and density, both of which are inherent physical properties derived from the molecular composition of the matrix particle. The hardness must be greater than that of the sample being pulverized, with higher hardness values being more effective at disrupting hard and brittle cell membranes. Density is defined as mass per unit volume, and by Newton’s 2nd Law, F=ma –it follows that a denser matrix particle will generate higher force and more effectively disrupt tough cell walls or hard samples with dense extracellular matrices (ie., seeds, spores, insects.) Hardness and density values help optimize lysis efficiency while preserving the integrity of the analytes of interest.
A word about knoop and mohs
Materials chosen for FastPrep® Lysing Matrix products are carefully selected based on several criteria. As discussed above, the hardness value is a key characteristic and is evaluated by standard assays, which are reported for each of our quality reagents. The Knoop hardness test defines mechanical hardness of brittle materials. A small indentation is made in the material surface using a diamond point applied with known force/time. The resulting indentation is measured using a microscope and scored with values from 100 to 1000, with increasing values being indicative of increased material hardness. The Mohs scale defines the scratch resistance of minerals, assessed as the ability of a harder material to scratch a softer material. Values range between 1 and 10, with talc being 1 and diamond 10. Hardness valuations used in engineering include Vickers and Rockwell, with higher numbers defining higher hardness.